Domaines de recherche
Etude des biolomécules (ADN, ARN, protéines) impliquées dans la maintenance de l'intégrité du génome (Réplication, Réparation, Recombinaison) chez l'organisme modèle Pyrococcus abyssi :
- Expression & Purification des protéines ciblées.
- Détermination des activités enzymatiques associés à ces protéines.
- Description des mécanismes biophysiques sous-jascents au sein des assemblages biologiques type :
- ADN : Protéine
- ARN : Protéine
- Protéine : Protéine
Sites Web
Labratoire de Microbiologie des Environnements Extrêmes (LM2E) : http://wwz.ifremer.fr/umr6197
Groupement de Recherche (GdR) Archaea : http://lbbe-dmz.univ-lyon1.fr/spip_archaea/spip.php?rubrique17
Société Française de Biochimie & de Biologie Moléculaire (SFBBM) : http://www.sfbbm.fr/
Bibliographie
2024
McTeer Logan, Moalic Yann, Cueff-Gauchard Valerie, Catchpole Ryan, Hogrel Gaëlle, Lu Yang, Laurent Sebastien, Hemon Marie, Aubé Johanne, Leroy Elodie, Roussel Erwan, Oberto Jacques, Flament Didier, Dulermo Remi (2024). Cooperation between two modes for DNA replication initiation in the archaeon Thermococcus barophilus . Mbio , 15(4), 15p. Publisher's official version : https://doi.org/10.1128/mbio.03200-23 , Open Access version : https://archimer.ifremer.fr/doc/00883/99483/
2023
Batour Maria, Laurent Sebastien, Moalic Yann, Chamieh Hala, Taha Samir, Jebbar Mohamed (2023). The secretome of Thermococcus barophilus in the presence of carbohydrates and the potential role of the TrmBL4 regulator . Environmental Microbiology Reports , 15(6), 530-544 . Publisher's official version : https://doi.org/10.1111/1758-2229.13186 , Open Access version : https://archimer.ifremer.fr/doc/00848/96003/
Madru Clément, Martínez-Carranza Markel, Laurent Sebastien, Alberti Alessandra C., Chevreuil Maelenn, Raynal Bertrand, Haouz Ahmed, Le Meur Rémy A., Delarue Marc, Henneke Ghislaine, Flament Didier, Krupovic Mart, Legrand Pierre, Sauguet Ludovic (2023). DNA-binding mechanism and evolution of replication protein A . Nature Communications , 14(1), 2326 (14p.) . Publisher's official version : https://doi.org/10.1038/s41467-023-38048-w , Open Access version : https://archimer.ifremer.fr/doc/00834/94613/
2022
Hogrel Gaëlle, Marino‐puertas Laura, Laurent Sebastien, Ibrahim Ziad, Covès Jacques, Girard Eric, Gabel Frank, Fenel Daphna, Daugeron Marie‐claire, Clouet‐d'Orval Béatrice, Basta Tamara, Flament Didier, Franzetti Bruno (2022). Characterization of a small tRNA ‐binding protein that interacts with the archaeal proteasome complex . Molecular Microbiology , 118(1-2), 16-29 . Publisher's official version : https://doi.org/10.1111/mmi.14948 , Open Access version : https://archimer.ifremer.fr/doc/00779/89070/
2021
Hajj Mirna, Langendijk-Genevaux Petra, Batista Manon, Quentin Yves, Laurent Sebastien, Capeyrou Régine, Abdel-Razzak Ziad, Flament Didier, Chamieh Hala, Fichant Gwennaele, Clouet-D’orval Béatrice, Bouvier Marie (2021). Phylogenetic Diversity of Lhr Proteins and Biochemical Activities of the Thermococcales aLhr2 DNA/RNA Helicase . Biomolecules , 11(7), 950 (23p.) . Publisher's official version : https://doi.org/10.3390/biom11070950 , Open Access version : https://archimer.ifremer.fr/doc/00702/81418/
2020
Prado Enora, Colas Florent, Laurent Sebastien, Tardivel Morgan, Evrard Justine, Forest Bertrand, Bocher Alan, Rouxel Justin (2020). Toward a SPR imaging in situ system to detect marine biotoxin . Michael T. Canva, Ambra Giannetti, Hatice Altug, Julien Moreau (Eds). Proceedings Volume 11361, Biophotonics in Point-of-Care; 113610J (2020). 13p.
Phung Duy Khanh, Etienne Clarisse, Batista Manon, Langendijk-Genevaux Petra, Moalic Yann, Laurent Sebastien, Liuu Sophie, Morales Violette, Jebbar Mohamed, Fichant Gwennaele, Bouvier Marie, Flament Didier, Clouet-D’orval Béatrice (2020). RNA processing machineries in Archaea: the 5′-3′ exoribonuclease aRNase J of the β-CASP family is engaged specifically with the helicase ASH-Ski2 and the 3′-5′ exoribonucleolytic RNA exosome machinery . Nucleic Acids Research , 48(7), 3832-3847 . Publisher's official version : https://doi.org/10.1093/nar/gkaa052 , Open Access version : https://archimer.ifremer.fr/doc/00610/72250/
2018
Lemor Melanie, Kong Ziqing, Henry Etienne, Brizard Raphael, Laurent Sebastien, Bosse Audrey, Henneke Ghislaine (2018). Differential Activities of DNA Polymerases in Processing Ribonucleotides during DNA Synthesis in Archaea . Journal Of Molecular Biology , 430(24), 4908-4924 . Publisher's official version : https://doi.org/10.1016/j.jmb.2018.10.004 , Open Access version : https://archimer.ifremer.fr/doc/00464/57603/
Hogrel Gaelle, Lu Yang, Laurent Sebastien, Henry Etienne, Etienne Clarisse, Duy Khanh Phung , Dulermo Remi, Bosse Audrey, Pluchon Pierre Francois, Clouet-D'Orval Beatrice, Flament Didier (2018). Physical and functional interplay between PCNA DNA clamp and Mre11–Rad50 complex from the archaeon Pyrococcus furiosus . Nucleic Acids Research , 46(11), 5651-5663 . Publisher's official version : https://doi.org/10.1093/nar/gky322 , Open Access version : https://archimer.ifremer.fr/doc/00439/55092/
2016
Colas Florent, Crassous Marie-Pierre, Laurent Sebastien, Litaker Richard W., Rinnert Emmanuel, Le Gall Erwan, Lunven Michel, Delauney Laurent, Compere Chantal (2016). A surface plasmon resonance system for the underwater detection of domoic acid . Limnology And Oceanography-methods , 14(7), 456-465 . Publisher's official version : https://doi.org/10.1002/lom3.10104 , Open Access version : https://archimer.ifremer.fr/doc/00324/43481/
2014
Laurent Sebastien, Richard Adrien, Mulner-Lorillon Odile, Morales Julia, Flament Didier, Glippa Virginie, Bourdon Jeremie, Gosselin Pauline, Siegel Anne, Cormier Patrick, Belle Robert (2014). Modelization of the regulation of protein synthesis following fertilization in sea urchin shows requirement of two processes: a destabilization of elF4E:4E-BP complex and a great stimulation of the 4E-BP-degradation mechanism, both rapamycin-sensitive . Frontiers In Genetics , 5(117), 1-10 . Publisher's official version : https://doi.org/10.3389/fgene.2014.00117 , Open Access version : https://archimer.ifremer.fr/doc/00382/49341/
2013
Pluchon Pierre Francois, Fouqueau Thomas, Creze Christophe, Laurent Sebastien, Briffotaux Julien, Hogrel Gaelle, Palud Adeline, Henneke Ghislaine, Godfroy Anne, Hausner Winfried, Thomm Michael, Nicolas Jacques, Flament Didier (2013). An Extended Network of Genomic Maintenance in the Archaeon Pyrococcus abyssi Highlights Unexpected Associations between Eucaryotic Homologs . Plos One , 8(11), - . Publisher's official version : https://doi.org/10.1371/journal.pone.0079707 , Open Access version : https://archimer.ifremer.fr/doc/00171/28198/
2012
Creze Christophe, Ligabue Alessio, Laurent Sebastien, Lestini Roxane, Laptenok Sergey P., Khun Joelle, Vos Marten H., Czjzek Mirjam, Myllykallio Hannu, Flament Didier (2012). Modulation of the Pyrococcus abyssi NucS Endonuclease Activity by Replication Clamp at Functional and Structural Levels . Journal Of Biological Chemistry , 287(19), 15648-15660 . https://doi.org/10.1074/jbc.M112.346361
2010
Colas Florent, Crassous Marie-Pierre, Laurent Sebastien, Dreanno Catherine, Compere Chantal (2010). New Approach For The Detection Of Toxic Algae Species . 14th International Conference on Harmful Algae. Crete, Greece, 1–5 November 2010 . https://archimer.ifremer.fr/doc/00089/19995/
Castrec Benoit, Laurent Sebastien, Henneke Ghislaine, Flament Didier, Raffin Jean-Paul (2010). The Glycine-Rich Motif of Pyrococcus abyssi DNA Polymerase D Is Critical for Protein Stability . Journal Of Molecular Biology , 396(4), 840-848 . Publisher's official version : https://doi.org/10.1016/j.jmb.2010.01.006 , Open Access version : https://archimer.ifremer.fr/doc/00002/11293/
2009
Laurent Sebastien, Colas Florent, Hamelin Muriel, Crassous Marie-Pierre, Antoine Elisabeth, Lehaitre Michel, Compere Chantal (2009). Toward Detection of Harmful Algae Blooms by in situ Surface Plasmon Resonance Spectroscopy . Sensors Applications Symposium, 2009. SAS 2009. IEEE , 29-33 . Publisher's official version : https://doi.org/10.1109/SAS.2009.4801771 , Open Access version : https://archimer.ifremer.fr/doc/00000/6830/
Colas Florent, Laurent Sebastien, Lehaitre Michel, Hamelin Muriel, Crassous Marie-Pierre, Antoine Elisabeth, Dreanno Catherine, Deuff Albert, Compere Chantal (2009). Toward in situ detection of algae species . Oceans 2009 - Europe, Vols 1 And 2 , 715-717 . Publisher's official version : https://doi.org/http://dx.doi.org/10.1109/OCEANSE.2009.5278172 , Open Access version : https://archimer.ifremer.fr/doc/00107/21802/
2008
Colas Florent, Compere Chantal, Geli Louis, Gentien Patrick, Gonzalez Jean-Louis, Laurent Sebastien, Lehaitre Michel, Lemoine Lionel, Le Traon Pierre-Yves, Le Hir Pierre, Peron Olivier, Rinnert Emmanuel, Rolin Jean-Francois, Salvetat Florence, Sarradin Pierre-Marie (2008). Essai de Prospective - Synthèse - Groupe Capteurs . ERT/IC – 08- 45 .