Etienne Henry

CNRS Research Engineer

Laboratoire de Microbiologie des Environnements Extrêmes (PDG-REM-BEEP-LMEE)

Contact form

+33 (0)2 98 22 45 26

Employer : CNRS

Site : Brest

Postal address : Centre Bretagne - ZI de la Pointe du Diable - CS 10070 - 29280 Plouzané


ORCID orcid icon : 0000-0002-2547-3467

ResearcherID : J-8118-2014

Research Interests

Molecular mechanisms of adaptation to extreme conditions of life - DNA Replication and repair in hyperthermophilic Archaea from deep hydrothermal vents - Functional characterization of DNA polymerases at high temperature
Biophysical methods for the study of molecular interactions - Fluorescence spectroscopy - FRET - Fluorescence anisotropy - Fluorescence microscopy - Cell imaging - Single molecule imaging - Image and data analysis - Python.


National and international programs

 Mission officer for the research infrastructure France Bio-Imaginig, French Node of the European Research Infrastructure Consortium Euro-BioImaging (EuBI ERIC).
France-BioImaging is a National Infrastructure in Biology and Health (INBS) laureate in 2011 of the national Program “Investissements d’Avenir” (PIA-ANR) in the field of biological imaging. FBI is at the crossroads between molecular and cell biology, biophysics and engineering, mathematics and informatics. This unique coordinated infrastructure gathers several large biological imaging facilities and laboratories specializing in R&D for imaging in 6 local and one transversal Nodes.




Carré Lorenzo, Henneke Ghislaine, Henry Etienne, Flament Didier, Girard Éric, Franzetti Bruno DNA Polymerization in Icy Moon Abyssal Pressure Conditions . Astrobiology IN PRESS . Publisher's official version : , Open Access version :


Fauvarque Olivier, Toffin Laurent, Henry Etienne, Rouxel Justin, Tardivel Morgan (2022). Expérimentation d'un fluorimètre dédié à l'observation des organismes méthanogènes dans les grands fonds océaniques . OPTIQUE Nice 2022 - Congrès de la Société Française d'Optique. 04-08 juillet 2022, Nice .
Fauvarque Olivier, Toffin Laurent, Henry Etienne, Tardivel Morgan, Rouxel Justin (2022). Expérimentation d'un fluorimètre dédié à l'observation des organismes méthanogènes dans les grands fonds océaniques . RBPGO9 - les 9èmes Rencontres Biologie-Physique du Grand Ouest 2022. 22-24 juin 2022, Brest .
Vialle Lea, Henry Etienne, Henneke Ghislaine (2022). Real-time measurement of ribonucleotide removal from DNA at high temperature . Single-Molecule Biophysics - The 2nd Single-Molecule Biophysics meeting. Sunday, February 27 – Friday, Mar 4 2022, Les Houches School of Physics, France .


Henry Etienne, Flament Didier, Henneke Ghislaine, Bouzigues Cedric, Coudreuse Damien, Griscom Laurent, Deprez Eric (2020). High-temperature single-molecule imaging to study the genome integrity in hyperthermophilic Archaea . The 1st Single-Molecule Biophysics Workshop. February 2, 2020 - February 7, 2020, Les Houches - School of Physics, France .


Raia Pierre, Carroni Marta, Henry Etienne, Pehau-Arnaudet Gerard, Brule Sebastien, Beguin Pierre, Henneke Ghislaine, Lindahl Erik, Delarue Marc, Sauguet Ludovic (2019). Structure of the DP1–DP2 PolD complex bound with DNA and its implications for the evolutionary history of DNA and RNA polymerases . Plos Biology , 17(1), e3000122 (28p.) . Publisher's official version : , Open Access version :


Lemor Melanie, Kong Ziqing, Henry Etienne, Brizard Raphael, Laurent Sebastien, Bosse Audrey, Henneke Ghislaine (2018). Differential Activities of DNA Polymerases in Processing Ribonucleotides during DNA Synthesis in Archaea . Journal Of Molecular Biology , 430(24), 4908-4924 . Publisher's official version : , Open Access version :
Hogrel Gaelle, Lu Yang, Laurent Sebastien, Henry Etienne, Etienne Clarisse, Duy Khanh Phung , Dulermo Remi, Bosse Audrey, Pluchon Pierre Francois, Clouet-D'Orval Beatrice, Flament Didier (2018). Physical and functional interplay between PCNA DNA clamp and Mre11–Rad50 complex from the archaeon Pyrococcus furiosus . Nucleic Acids Research , 46(11), 5651-5663 . Publisher's official version : , Open Access version :


Ralec Celine, Henry Etienne, Lemor Melanie, Killelea Tom, Henneke Ghislaine (2017). Calcium-driven DNA synthesis by a high-fidelity DNA polymerase . Nucleic Acids Research , 45(21), 12425-12440 . Publisher's official version : , Open Access version :
Henry Etienne, Henneke Ghislaine, Flament Didier (2017). Real-time fluorescence assays to monitor DNA polymerase activities . Biophysical Society 61st Annual Meeting. February 11-15 2017, New Orleans, Louisiana .


Nguyen N. -H., Bogliotti N., Chennoufi R., Henry Etienne, Tauc P., Salas E., Roman L. J., Slama-Schwok A., Deprez E., Xie J. (2016). Convergent synthesis and properties of photoactivable NADPH mimics targeting nitric oxide synthases . Organic & Biomolecular Chemistry , 14(40), 9519-9532 .
Qin Wei, Bazeille Nicolas, Henry Etienne, Zhang Bo, Deprez Eric, Xi Xu-Guang (2016). Mechanistic insight into cadmium-induced inactivation of the Bloom protein . Scientific Reports , 6(26225), 12p. Publisher's official version : , Open Access version :
Chennoufi Rahima, Bougherara Houcine, Gagey-Eilstein Nathalie, Dumat Blaise, Henry Etienne, Subra Frederic, Bury-Mone Stephanie, Mahuteau-Betzer Florence, Tauc Patrick, Teulade-Fichou Marie-Paule, Deprez Eric (2016). Mitochondria-targeted Triphenylamine Derivatives Activatable by Two-Photon Excitation for Triggering and Imaging Cell Apoptosis . Scientific Reports , 6(21458), 12p. Publisher's official version : , Open Access version :


Hamoudi Mounira Cherifa, Henry Etienne, Zerrouk Naima, Scherman Daniel, Arnaud Philippe, Deprez Eric, Escriou Virginie (2015). Enhancement of siRNA lipid-based vector stability and siRNA integrity in human serum with addition of anionic polymer adjuvant . Journal Of Drug Delivery Science And Technology , 26, 1-9 .
Diaw A. K. D., Gningue-Sall D., Yassar A., Brochon J. -C, Henry Etienne, Aaron J. -J (2015). An experimental study of the electronic absorption and fluorescence spectral properties of new p-substituted-N-phenylpyrroles and their electrosynthesized polymers . Spectrochimica Acta Part A-molecular And Biomolecular Spectroscopy , 135, 1107-1114 .
Chennoufi R., Bougherara H., Gagey-Eilstein N., Dumat B., Henry Etienne, Subra F., Mahuteau-Betzer F., Tauc P., Teulade-Fichou M-P., Deprez E. (2015). Differential Behaviour of Cationic Triphenylamine Derivatives in Fixed and Living Cells: Triggering and Imaging Cell Death . Chemical Communications , 51(80), 14881-14884 .


Henry Etienne, Deprez Eric, Brochon Jean-Claude (2014). Maximum entropy analysis of data simulations and practical aspects of time-resolved fluorescence measurements in the study of molecular interactions . Journal Of Molecular Structure , 1077, 77-86 .
Mbemba Gladys, Henry Etienne, Delelis Olivier, Bouger Marie-Christine, Buckle Malcolm, Mouscadet Jean-Francois, Hazan Uriel, Leh Herve, Bury-Mone Stephanie (2014). Biochemical properties of the xenotropic murine leukemia virus-related virus integrase . Biochimie , 107(Part. B), 300-309 .
Delplanque Aleksandra, Henry Etienne, Lautru Joseph, Leh Herve, Buckle Malcolm, Nogues Claude (2014). UV/ozone surface treatment increases hydrophilicity and enhances functionality of SU-8 photoresist polymer . Applied Surface Science , 314, 280-285 .
Lo Cheikh, Doucoure Baba Issa, Aaron Jean-Jacques, Svoboda Jiri, Kozmik Vaclav, Brochon Jean-Claude, Henry Etienne, Maurel Francois, Capochichi Martine (2014). Synthesis and spectral properties of new fluorescent alkoxy-substituted thieno[3,2-b]indole derivatives . Spectrochimica Acta Part A-molecular And Biomolecular Spectroscopy , 120, 47-54 .
Rouaud Florian, Romero-Perez Miguel, Wang Huan, Lobysheva Irina, Ramassamy Booma, Henry Etienne, Tauc Patrick, Giacchero Damien, Boucher Jean-Luc, Deprez Eric, Rocchi Stephane, Slama-Schwok Anny (2014). Regulation of NADPH-dependent Nitric Oxide and reactive oxygen species signalling in endothelial and melanoma cells by a photoactive NADPH analogue . Oncotarget , 5(21), 10650-1066 .


Li Jiayao, Henry Etienne, Wang Lanmei, Delelis Olivier, Wang Huan, Simon Francoise, Tauc Patrick, Brochon Jean-Claude, Zhao Yunlong, Deprez Eric (2012). Comparative Study of the Fatty Acid Binding Process of a New FABP from Cherax quadricarinatus by Fluorescence Intensity, Lifetime and Anisotropy . Plos One , 7(12), - . Publisher's official version : , Open Access version :
Li Yun, Wang Huan, Tarus Bogdan, Perez Miguel Romero, Morellato Laurence, Henry Etienne, Berka Vladimir, Tsai Ah-Lim, Ramassamy Booma, Dhimane Hamid, Dessy Chantal, Tauc Patrick, Boucher Jean-Luc, Deprez Eric, Slama-Schwok Anny (2012). Rational design of a fluorescent NADPH derivative imaging constitutive nitric-oxide synthases upon two-photon excitation . Proceedings Of The National Academy Of Sciences Of The United States Of America , 109(31), 12526-12531 .
Henry Etienne, Li Yun, Wang Huan, Tarus Bogdan, Dessy Chantal, Tauc Patrick, Deprez Eric, Boucher Jean-Luc, Slama-Schwok Anny (2012). A Two-Photon Absorption Probe Mimicking NADPH that Inhibits the Nitric Oxyde Synthase . Biophysical Society 56st Annual Meeting. February 25-29 2012, San Diego, California .


Henry Etienne, Dif Aurelien, Schmutz Marc, Legoff Loic, Amblard Francois, Marchi-Artzner Valerie, Artzner Franck (2011). Crystallization of Fluorescent Quantum Dots within a Three-Dimensional Bio-Organic Template of Actin Filaments and Lipid Membranes . Nano Letters , 11(12), 5443-5448 .
Henry Etienne, Xu-Li Yun, Wang Huan, Tauc Patrick, Boucher Jean-Luc, Slama-Schwok Anny, Deprez Eric (2011). Targeting the NADPH Binding Site of Nitric Oxide Synthase by a Ligand with Two-Photon Absorption Properties . Biophysical Society 55st Annual Meeting. March 5-9 2011, Baltimore, Maryland .
Henry Etienne, Dif A., Schmutz M., Legoff L., Amblard F., Marchi V., Artzner F. (2011). 3D arrays on quantum dots embedded within a bio-inspired template studied by SAXS . Synchrotron Soleil Highlights , 34-35 . Open Access version :


Carayon Kevin, Leh Herve, Henry Etienne, Simon Francoise, Mouscadet Jean-Francois, Deprez Eric (2010). A cooperative and specific DNA-binding mode of HIV-1 integrase depends on the nature of the metallic cofactor and involves the zinc-containing N-terminal domain . Nucleic Acids Research , 38(11), 3692-3708 . Publisher's official version : , Open Access version :
Li Chun, Henry Etienne, Mani Naresh Kumar, Tang Jie, Brochon Jean-Claude, Deprez Eric, Xie Juan (2010). Click Chemistry to Fluorescent Amino Esters: Synthesis and Spectroscopic Studies . European Journal Of Organic Chemistry , (12), 2395-2405 .
Li Na, Henry Etienne, Guiot Elvire, Rigolet Pascal, Brochon Jean-Claude, Xi Xu-Guang, Deprez Eric (2010). Multiple Escherichia coli RecQ Helicase Monomers Cooperate to Unwind Long DNA Substrates A FLUORESCENCE CROSS-CORRELATION SPECTROSCOPY STUDY . Journal Of Biological Chemistry , 285(10), 6922-6936 .


Henry Etienne, Na Li, Dong Chaoqing, Ren Jicun, Deprez Eric, Brochon Jean-Claude (2009). Multi-exponential Luminescence Decay of Non-blinking CdTe Quantum dots upon one and two photon excitation . Biophysical Society 53st Annual Meeting. February 28-March 4 2009, Boston, Massachusetts .


Dif Aurelien, Henry Etienne, Artzner Franck, Baudy-Floc'h Michele, Schmutz Marc, Dahan Maxinne, Marchi-Artzner Valrie (2008). Interaction between water-soluble peptidic CdSe/ZnS nanocrystals and membranes: Formation of hybrid vesicles and condensed lamellar phases . Journal Of The American Chemical Society , 130(26), 8289-8296 .


Taberlet Nicolas, Richard Patrick, Henry Etienne, Delannay Renaud (2004). The growth of a Super Stable Heap: An experimental and numerical study . Europhysics Letters , 68(4), 515-521 .