Didier Flament

Didier Flament

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Employer : Ifremer

Site : Brest

Postal address : Centre Bretagne - ZI de la Pointe du Diable - CS 10070 - 29280 Plouzané

URL : https://annuaire.ifremer.fr/cv/16038/en/

ORCID orcid icon : 0000-0002-9847-2342



McTeer Logan, Moalic Yann, Cueff-Gauchard Valerie, Catchpole Ryan, Hogrel Gaëlle, Lu Yang, Laurent Sebastien, Hemon Marie, Aubé Johanne, Leroy Elodie, Roussel Erwan, Oberto Jacques, Flament Didier, Dulermo Remi (2024). Cooperation between two modes for DNA replication initiation in the archaeon Thermococcus barophilus . Mbio , 15(4), 15p. Publisher's official version : https://doi.org/10.1128/mbio.03200-23 , Open Access version : https://archimer.ifremer.fr/doc/00883/99483/
Carré Lorenzo, Henneke Ghislaine, Henry Etienne, Flament Didier, Girard Éric, Franzetti Bruno (2024). DNA Polymerization in Icy Moon Abyssal Pressure Conditions . Astrobiology , 24(2), 151-162 . Publisher's official version : https://doi.org/10.1089/ast.2021.0201 , Open Access version : https://archimer.ifremer.fr/doc/00816/92758/
Batista Manon, Langendijk-Genevaux Petra, Kwapisz Marta, Canal Isabelle, Phung Duy khanh, Plassart Laura, Capeyrou Régine, Moalic Yann, Jebbar Mohamed, Flament Didier, Fichant Gwennaele, Bouvier Marie, Clouet-D’orval Béatrice (2024). Evolutionary and functional insights into the Ski2-like helicase family in Archaea: a comparison of Thermococcales ASH-Ski2 and Hel308 activities . Nar Genomics And Bioinformatics , 6(1), lqae026 (16p.) . Publisher's official version : https://doi.org/10.1093/nargab/lqae026 , Open Access version : https://archimer.ifremer.fr/doc/00883/99491/


Mottez Clémence, Puech Romain, Flament Didier, Myllykallio Hannu Structuring effects of archaeal replication origins . bioRxiv IN PRESS . Publisher's official version : https://doi.org/10.1101/2023.11.15.567178 , Open Access version : https://archimer.ifremer.fr/doc/00873/98479/
Madru Clément, Martínez-Carranza Markel, Laurent Sebastien, Alberti Alessandra C., Chevreuil Maelenn, Raynal Bertrand, Haouz Ahmed, Le Meur Rémy A., Delarue Marc, Henneke Ghislaine, Flament Didier, Krupovic Mart, Legrand Pierre, Sauguet Ludovic (2023). DNA-binding mechanism and evolution of replication protein A . Nature Communications , 14(1), 2326 (14p.) . Publisher's official version : https://doi.org/10.1038/s41467-023-38048-w , Open Access version : https://archimer.ifremer.fr/doc/00834/94613/


Coste Franck, Goffinont Stéphane, Cros Julien, Gaudon Virginie, Guerin Martine, Garnier Norbert, Confalonieri Fabrice, Flament Didier, Suskiewicz Marcin Josef, Castaing Bertrand (2022). Structural and functional determinants of the archaeal 8-oxoguanine-DNA glycosylase AGOG for DNA damage recognition and processing . Nucleic Acids Research , 50(19), 11072-11092 . Publisher's official version : https://doi.org/10.1093/nar/gkac932 , Open Access version : https://archimer.ifremer.fr/doc/00800/91216/
Hogrel Gaëlle, Marino‐puertas Laura, Laurent Sebastien, Ibrahim Ziad, Covès Jacques, Girard Eric, Gabel Frank, Fenel Daphna, Daugeron Marie‐claire, Clouet‐d'Orval Béatrice, Basta Tamara, Flament Didier, Franzetti Bruno (2022). Characterization of a small tRNA ‐binding protein that interacts with the archaeal proteasome complex . Molecular Microbiology , 118(1-2), 16-29 . Publisher's official version : https://doi.org/10.1111/mmi.14948 , Open Access version : https://archimer.ifremer.fr/doc/00779/89070/


Hajj Mirna, Langendijk-Genevaux Petra, Batista Manon, Quentin Yves, Laurent Sebastien, Capeyrou Régine, Abdel-Razzak Ziad, Flament Didier, Chamieh Hala, Fichant Gwennaele, Clouet-D’orval Béatrice, Bouvier Marie (2021). Phylogenetic Diversity of Lhr Proteins and Biochemical Activities of the Thermococcales aLhr2 DNA/RNA Helicase . Biomolecules , 11(7), 950 (23p.) . Publisher's official version : https://doi.org/10.3390/biom11070950 , Open Access version : https://archimer.ifremer.fr/doc/00702/81418/


Hogrel Gaelle, Lu Yang, Alexandre Nicolas, Bossé Audrey, Dulermo Remi, Ishino Sonoko, Ishino Yoshizumi, Flament Didier (2020). Role of RadA and DNA Polymerases in Recombination-Associated DNA Synthesis in Hyperthermophilic Archaea . Biomolecules , 10(7), 1045 (17p.) . Publisher's official version : https://doi.org/10.3390/biom10071045 , Open Access version : https://archimer.ifremer.fr/doc/00641/75274/
Phung Duy Khanh, Etienne Clarisse, Batista Manon, Langendijk-Genevaux Petra, Moalic Yann, Laurent Sebastien, Liuu Sophie, Morales Violette, Jebbar Mohamed, Fichant Gwennaele, Bouvier Marie, Flament Didier, Clouet-D’orval Béatrice (2020). RNA processing machineries in Archaea: the 5′-3′ exoribonuclease aRNase J of the β-CASP family is engaged specifically with the helicase ASH-Ski2 and the 3′-5′ exoribonucleolytic RNA exosome machinery . Nucleic Acids Research , 48(7), 3832-3847 . Publisher's official version : https://doi.org/10.1093/nar/gkaa052 , Open Access version : https://archimer.ifremer.fr/doc/00610/72250/
Henry Etienne, Flament Didier, Henneke Ghislaine, Bouzigues Cedric, Coudreuse Damien, Griscom Laurent, Deprez Eric (2020). High-temperature single-molecule imaging to study the genome integrity in hyperthermophilic Archaea . The 1st Single-Molecule Biophysics Workshop. February 2, 2020 - February 7, 2020, Les Houches - School of Physics, France .


Compere Chantal, Dreanno Catherine, Colas Florent, Delauney Laurent, Henneke Ghislaine, Flament Didier (2018). In situ Biosensors and associated Diagnose Methods . ASIMO Workshop - Autonomous Systems for Integrated Marine and Maritime Observations in Coastal areas. 17-19 september 2018, Las Palmas, Canary Islands, Spain.
Hogrel Gaelle, Lu Yang, Laurent Sebastien, Henry Etienne, Etienne Clarisse, Duy Khanh Phung , Dulermo Remi, Bosse Audrey, Pluchon Pierre Francois, Clouet-D'Orval Beatrice, Flament Didier (2018). Physical and functional interplay between PCNA DNA clamp and Mre11–Rad50 complex from the archaeon Pyrococcus furiosus . Nucleic Acids Research , 46(11), 5651-5663 . Publisher's official version : https://doi.org/10.1093/nar/gky322 , Open Access version : https://archimer.ifremer.fr/doc/00439/55092/
Birien Tiphaine, Thiel Axel, Henneke Ghislaine, Flament Didier, Moalic Yann, Jebbar Mohamed (2018). Development of an Effective 6-Methylpurine Counterselection Marker for Genetic Manipulation in Thermococcus barophilus . Genes , 9(2), 77 (13p.) . Publisher's official version : https://doi.org/10.3390/genes9020077 , Open Access version : https://archimer.ifremer.fr/doc/00425/53679/


Henry Etienne, Henneke Ghislaine, Flament Didier (2017). Real-time fluorescence assays to monitor DNA polymerase activities . Biophysical Society 61st Annual Meeting. February 11-15 2017, New Orleans, Louisiana .


Sarradin Pierre-Marie, Sarrazin Jozee, Flament Didier (2016). Unité de Recherche Etude des Ecosystèmes Profonds - Rapport annuel d'activités 2015 . REM/EEP/16-03 .


Godfroy Anne, Jebbar Mohamed, Flament Didier, Henneke Ghislaine (2014). Marine ocean depths in the laboratory . Biofutur , (360), 36-38 .
Laurent Sebastien, Richard Adrien, Mulner-Lorillon Odile, Morales Julia, Flament Didier, Glippa Virginie, Bourdon Jeremie, Gosselin Pauline, Siegel Anne, Cormier Patrick, Belle Robert (2014). Modelization of the regulation of protein synthesis following fertilization in sea urchin shows requirement of two processes: a destabilization of elF4E:4E-BP complex and a great stimulation of the 4E-BP-degradation mechanism, both rapamycin-sensitive . Frontiers In Genetics , 5(117), 1-10 . Publisher's official version : https://doi.org/10.3389/fgene.2014.00117 , Open Access version : https://archimer.ifremer.fr/doc/00382/49341/
Thiel Axel, Michoud Gregoire, Moalic Yann, Flament Didier, Jebbar Mohamed (2014). Genetic Manipulations of the Hyperthermophilic Piezophilic Archaeon Thermococcus barophilus . Applied And Environmental Microbiology , 80(7), 2299-2306 . Publisher's official version : https://doi.org/10.1128/AEM.00084-14 , Open Access version : https://archimer.ifremer.fr/doc/00187/29818/


Pluchon Pierre Francois, Fouqueau Thomas, Creze Christophe, Laurent Sebastien, Briffotaux Julien, Hogrel Gaelle, Palud Adeline, Henneke Ghislaine, Godfroy Anne, Hausner Winfried, Thomm Michael, Nicolas Jacques, Flament Didier (2013). An Extended Network of Genomic Maintenance in the Archaeon Pyrococcus abyssi Highlights Unexpected Associations between Eucaryotic Homologs . Plos One , 8(11), - . Publisher's official version : https://doi.org/10.1371/journal.pone.0079707 , Open Access version : https://archimer.ifremer.fr/doc/00171/28198/
Leulliot N., Quevillon-Cheruel S., Graille M., Geslin Claire, Flament Didier, Le Romancer Marc, Van Tilbeurgh H. (2013). Crystal Structure of PAV1-137: A Protein from the Virus PAV1 That Infects Pyrococcus abyssi . Archaea-an International Microbiological Journal , (ID 568053), 1-5 . Publisher's official version : https://doi.org/10.1155/2013/568053 , Open Access version : https://archimer.ifremer.fr/doc/00134/24562/


Creze Christophe, Ligabue Alessio, Laurent Sebastien, Lestini Roxane, Laptenok Sergey P., Khun Joelle, Vos Marten H., Czjzek Mirjam, Myllykallio Hannu, Flament Didier (2012). Modulation of the Pyrococcus abyssi NucS Endonuclease Activity by Replication Clamp at Functional and Structural Levels . Journal Of Biological Chemistry , 287(19), 15648-15660 . https://doi.org/10.1074/jbc.M112.346361


Compere Chantal, Dreanno Catherine, Colas Florent, Henneke Ghislaine, Flament Didier, Delauney Laurent (2011). Biocapteurs in situ et méthodes de diagnose associées .
Creze Christophe, Lestini Roxane, Kuehn Joelle, Ligabue Alessio, Becker Hubert F., Czjzek Mirjam, Flament Didier, Myllykallio Hannu (2011). Structure and function of a novel endonuclease acting on branched DNA substrates . Biochemical Society Transactions , 39, 145-149 . https://doi.org/10.1042/BST0390145


Groisillier Agnes, Herve Christiane, Jeudy Alexandra, Rebuffet Etienne, Pluchon Pierre Francois, Chevolot Yann, Flament Didier, Geslin Claire, Morgado Isabel M., Power Deborah, Branno Margherita, Moreau Herve, Michel Gurvan, Boyen Catherine, Czjzek Mirjam (2010). MARINE-EXPRESS: taking advantage of high throughput cloning and expression strategies for the post-genomic analysis of marine organisms . Microbial Cell Factories , 9, - . Publisher's official version : https://doi.org/10.1186/1475-2859-9-45 , Open Access version : https://archimer.ifremer.fr/doc/00011/12219/
Castrec Benoit, Laurent Sebastien, Henneke Ghislaine, Flament Didier, Raffin Jean-Paul (2010). The Glycine-Rich Motif of Pyrococcus abyssi DNA Polymerase D Is Critical for Protein Stability . Journal Of Molecular Biology , 396(4), 840-848 . Publisher's official version : https://doi.org/10.1016/j.jmb.2010.01.006 , Open Access version : https://archimer.ifremer.fr/doc/00002/11293/


Castrec Benoit, Rouillon Christophe, Henneke Ghislaine, Flament Didier, Querellou Joel, Raffin Jean-Paul (2009). Binding to PCNA in Euryarchaeal DNA Replication Requires Two PIP Motifs for DNA Polymerase D and One PIP Motif for DNA Polymerase B . Journal of Molecular Biology , 394(2), 209-218 . Publisher's official version : https://doi.org/10.1016/j.jmb.2009.09.044 , Open Access version : https://archimer.ifremer.fr/doc/00000/7317/
Ren Bin, Kuehn Joelle, Meslet-Cladiere Laurence, Briffotaux Julien, Norais Cedric, Lavigne Regis, Flament Didier, Ladenstein Rudolf, Myllykallio Hannu (2009). Structure and function of a novel endonuclease acting on branched DNA substrates . Embo Journal , 28(16), 2479-2489 . Publisher's official version : https://doi.org/10.1038/emboj.2009.192 , Open Access version : https://archimer.ifremer.fr/doc/00000/6807/


Le Breton Magali, Henneke Ghislaine, Norais C, Flament Didier, Myllykallio H, Querellou Joel, Raffin Jean-Paul (2007). The heterodimeric primase from the euryarchaeon Pyrococcus abyssi: A multifunctional enzyme for initiation and repair? Journal of Molecular Biology , 374(5), 1172-1185 . Publisher's official version : https://doi.org/10.1016/j.jmb.2007.10.015 , Open Access version : https://archimer.ifremer.fr/doc/00000/3520/
Mesiet Cladiere L, Norais Cédric, Kuhn Joelle, Briffotaux Julien, Sloostra Jerry W., Ferrari Elena, Hubscher Ulrich, Flament Didier, Myllykallio Hannu (2007). A novel proteomic approach identifies new interaction partners for proliferating cell nuclear antigen . Journal of Molecular Biology , 372(5), 1137-1148 . Publisher's official version : https://doi.org/10.1016/j.jmb.2007.06.056 , Open Access version : https://archimer.ifremer.fr/doc/00000/3297/
Flament Didier, Barbeyron Tristan, Jam Murielle, Potin Philippe, Czjzek Mirjam, Kloareg Bernard, Michel Gurvan (2007). Alpha-agarases define a new family of glycoside hydrolases, distinct from beta-agarase families . Applied and environmental microbiology , 73(14), 4691-4694 . Publisher's official version : https://doi.org/10.1128/AEM.00496-07 , Open Access version : https://archimer.ifremer.fr/doc/00000/2826/
Gras S, Chaumont V, Fernandez B, Carpentier P, Charrier Savournin F, Schmitt Sophie, Pineau C, Flament Didier, Hecker A, Forterre P, Armengaud J, Housset D (2007). Structural insights into a new homodimeric self-activated GTPase family . Embo reports , 8(6), 569-575 . Publisher's official version : https://doi.org/10.1038/sj.embor.7400958 , Open Access version : https://archimer.ifremer.fr/doc/00000/4724/
Rouillon Christophe, Henneke Ghislaine, Flament Didier, Querellou Joel, Raffin Jean-Paul (2007). DNA polymerase switching on homotrimeric PCNA at the replication fork of the euryarchaea Pyrococcus abyssi . Journal of Molecular Biology , 369(2), 343-355 . Publisher's official version : https://doi.org/10.1016/j.jmb.2007.03.054 , Open Access version : https://archimer.ifremer.fr/doc/00000/2638/
Geslin Claire, Gaillard Melusine, Flament Didier, Rouault Karen, Le Romancer Marc, Prieur Daniel, Erauso Gael (2007). Analysis of the first genome of a hyperthermophilic marine virus-like particle, PAV1, isolated from Pyrococcus abyssi . Journal of bacteriology , 189(12), 4510-4519 . Publisher's official version : https://doi.org/10.1128/JB.01896-06 , Open Access version : https://archimer.ifremer.fr/doc/00000/3536/


Prieur Daniel, Erauso Gael, Flament Didier, Gaillard Melusine, Geslin Claire, Gonnet Mathieu, Le Romancer Marc, Lucas Soizick, Forterrre Patrick (2006). Deep-sea thermococcales and their genetic elements: Plasmids and viruses . Extremophiles Methods In Microbiology , 35, 253-278 .


Henneke Ghislaine, Flament Didier, Hubscher Ulrich, Querellou Joel, Raffin Jean-Paul (2005). The hyperthermophilic euryarchaeota Pyrococcus abyssi likely requires the two DNA polymerases D and B for DNA replication . Journal of Molecular Biology , 350(1), 53-64 . Publisher's official version : https://doi.org/10.1016/j.jmb.2005.04.042 , Open Access version : https://archimer.ifremer.fr/doc/00000/423/


Prieur D, Erauso G, Geslin C, Lucas S, Gaillard M, Bidault A, Mattenet Ac, Rouault Karen, Flament Didier, Forterre P, Le Romancer M (2004). Genetic elements of Thermococcales . Biochemical Society Transactions , 32, 184-187 .


Cohen Gn, Barbe V, Flament Didier, Galperin M, Heilig R, Lecompte O, Poch O, Prieur D, Querellou Joel, Ripp R, Thierry Jc, Van Der Oost J, Weissenbach J, Zivanovic Y, Forterre P (2003). An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi . Molecular Microbiology , 47(6), 1495-1512 . https://doi.org/10.1046/j.1365-2958.2003.03381.x
Gueguen Yannick, Cadoret Jean-Paul, Flament Didier, Barreau-Roumiguiere C, Girardot Anne-Laure, Garnier Julien, Hoareau A, Bachere Evelyne, Escoubas Jean-Michel (2003). Immune gene discovery by expressed sequence tags generated from hemocytes of the bacteria-challenged oyster, Crassostrea gigas . Gene , 303, 139-145 . https://doi.org/10.1016/S0378-1119(02)01149-6


Henneke Ghislaine, Gueguen Yannick, Flament Didier, Azam Philippe, Querellou Joel, Dietrich Jacques, Hubscher Ulrich, Raffin Jean-Paul (2002). Replication factor C from the hyperthermophilic archaeon Pyrococcus abyssi does not need ATP hydrolysis for clamp-loading and contains a functionally conserved RFC PCNA-binding domain . Journal of Molecular Biology , 323(5), 795-810 . Publisher's official version : https://doi.org/10.1016/S0022-2836(02)01028-8 , Open Access version : https://archimer.ifremer.fr/doc/00000/675/
Rolland Jean-Luc, Gueguen Yannick, Flament Didier, Pouliquen Yann, Street Pfs, Dietrich Jacques (2002). Comment on "The first description of an archaeal hemicellulase: the xylanase from Thermococcus zilligii strain AN1": evidence that the unique N-terminal sequence proposed comes from a maltodextrin phosphorylase . Extremophiles , 6(4), 349-350 . https://doi.org/10.1007/s00792-001-0258-z
Chevalet Claude, Martin Patrice, Jouin Patrick, Flament Didier, Villeneuve Laure, Ky Chin-Long, Leroux Frederic, Lapegue Sylvie, Fabioux Caroline, Huvet Arnaud, Samain Jean-Francois, Escoubas Jean Michel (2002). Recueil des présentations du forum génomique. La Tremblade, 15-16 novembre 2001 . R.INT.TMSI/IDM/RIC/02-021 .


Gueguen Yannick, Rolland Jean-Luc, Lecompte O, Azam Philippe, Le Romancer Gisele, Flament Didier, Raffin Jean-Paul, Dietrich Jacques (2001). Characterization of two DNA polymerases from the hyperthermophilic euryarchaeon Pyrococcus abyssi . European Journal Of Biochemistry , 268(22), 5961-5969 . https://doi.org/10.1046/j.0014-2956.2001.02550.x
Zappa Sebastien, Rolland Jean-Luc, Flament Didier, Gueguen Yannick, Boudrant Joseph, Dietrich Jacques (2001). Characterization of a highly thermostable alkaline phosphatase from the euryarchaeon Pyrococcus abyssi . Applied and environmental microbiology , 67(10), 4504-4511 . Publisher's official version : https://doi.org/10.1128/AEM.67.10.4504-4511.2001 , Open Access version : https://archimer.ifremer.fr/doc/00000/1268/
Gueguen Yannick, Rolland Jean-Luc, Schroeck S, Flament Didier, Defretin S, Saniez Mh, Dietrich Jacques (2001). Characterization of the maltooligosyl trehalose synthase from the thermophilic archaeon Sulfolobus acidocaldarius . Fems Microbiology Letters , 194(2), 201-206 . https://doi.org/10.1111/j.1574-6968.2001.tb09470.x